Degree of hydrolization of gallotannins from Sumac (Rhus coriaria) on their anti-inflammatory potency in vitro

dc.contributor.authorRivas M., Alessandra
dc.contributor.colaboratorCardona, Jorge
dc.contributor.colaboratorDi lorio, Adriana
dc.contributor.colaboratorTalcott, Susanne
dc.coverage.spatialZamorano
dc.date.accessioned2019-11-26T03:46:25Z
dc.date.available2019-11-26T03:46:25Z
dc.date.issued2019
dc.description.abstractThe sumac is a Middle Eastern flavoring spice rich in polyphenols. Polyphenols are compounds present in plants, which according to many investigations have a positive effect on chronic diseases such as cancer. The objective of this study was to hydrolyze a sumac extract in three different levels and evaluate its anti-inflammatory effect in colon cancer cells (HT-29). Completely randomized designs were used for the rhodanine assay for four hours (0-240 min); the proliferation of HT-29 cells with three concentrations of sumac extract (1, 5 and 25 mg / L) and three levels of enzymatic hydrolysis (non-hydrolyzed, partially hydrolyzed and hydrolyzed), and reverse transcriptase polymerase chain reaction using the three levels of hydrolysis with one concentration of sumac extract (5 mg / L). The enzymatic hydrolysis carried out by tannase lasted between 90 and 120 minutes. It degraded the high molecular weight tannins and converted them to gallic acid, from which 34 g/L were obtained along with smaller gallotannins. In the evaluation of cell proliferation, the positive effects of the degree of hydrolysis and the different concentrations used were observed. There was no decrease in the expression of some inflammation markers (NF-ĸB, TNF-α and IL-1β), while better results were observed with the sample of hydrolyzed particles with the rest of molecular markers (VCAM-1, TRL4, IL -8 and COX-2). It is recommended to continue this study in vivo and with other cell lines.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://bdigital.zamorano.edu/handle/11036/6499
dc.language.isoeng
dc.publisherZamorano: Escuela Agrícola Panamericana, 2019.
dc.relation29 p.
dc.rightsCopyright Escuela Agrícola Panamericana, Zamorano, 2019
dc.rights.accessrightsopenAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/es
dc.subjectCell proliferation
dc.subjectInflammation markers
dc.subjectPolyphenols
dc.subjectTannase
dc.subjectMarcadores de inflamación
dc.subjectPolifenoles
dc.subjectProliferación celular
dc.subjectTanasa
dc.titleDegree of hydrolization of gallotannins from Sumac (Rhus coriaria) on their anti-inflammatory potency in vitro
dc.typeThesis
Archivos
Bloque original
Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
AGI-2019-T050.pdf
Tamaño:
930.49 KB
Formato:
Adobe Portable Document Format
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descripción: