Inactivation of Bacillus atrophaeus endospores using cold plasma treatment

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2019
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Zamorano: Escuela Agrícola Panamericana, 2019.
Resumen
Cold plasma treatment is a novel technology that is being investigated for its use in the food industry due to its ability to inactivate microorganisms, and keep certain characteristics of freshness in food products. The efficiency of the cold plasma treatment using Argon over Bacillus atrophaeus endospores was evaluated at different exposure times (30 s, 2, 4 and 6 min). Four concentrations (104 to 107 ) of B. atrophaeus endospores were inoculated on polyethylene plastic strips and treated inside a sterile sealed sample box filled with Argon. The samples were treated using a Dielectric Barrier Discharge Cold Plasma equipment with 60 kV. Results were collected using plate count method 24 and 48 h after treatment. A 1.8 Log CFU/ mL reduction of Bacillus atrophaeus endospores was achieved after 6 min of exposure using 60 kV. A standard operating procedure was elaborated for Bacillus atrophaeus spore inactivation using cold plasma. More research is needed to understand the effect of different gases over endospores of Bacillus atrophaeus
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Argon, Dielectric barrier discharge, Nonthermal technology, Pathogen control, Argon, Control esporulados, Descarga de barrera dieléctrica, Tecnología no térmica
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